Transformation is the uptake of plasmid DNA by E.Coli. Which of the following st

Question

Transformation is the uptake of plasmid DNA by E.Coli. Which of the following statements DOES NOT describe this technique?

a) Heat shock transformation is the use of high heat to allow plasmid DNA to enter the bacterial cell.

b) Electroporation is the use of current to make membranes leaky so that cells take up DNA molecules.

c) Competent cells must be cold so that the DNA stays inside the cells.

d) Nonselective media must be used to allow noncompetent cells to become competent cells and grow to log phase.

e) Transformed cells will grow and devide, each forming aq colony on the plate, indicative of a single transformation event.

Explanation / Answer

Transformation is a process where you introduce a foreign piece of DNA or a plasmid into a bacteria to transform the bacteria for a specific phenotype. It involves either heat shock where you briefly give a temperature shock to open the membrane transiently or electroporation where you provide current to make membrane leaky to allow the DNA to pass into the cell.

The answer is Nonselective media must be used to allow noncompetent cells to become competent cells and grow to log phase.

Nonselective media does not make a noncompetent cell to become competent rather incubating the noncompetent cells with calcium chloride will make it competent.

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