A mutant yeast strain can convert galactose to UDP-galactose, but is unable to c

Question

A mutant yeast strain can convert galactose to UDP-galactose, but is unable to carry out the next step of galactose metabolism because it cannot make the Gal10 enzyme. All of the following are possible explanations for this phenotype EXCEPT

A) A mutation in the protein-coding region of the GAL10 gene.

B) A deletion of the UAS of the GAL10 gene.

C) A deletion affecting the GAL4 protein-binding site upstream of the GAL10 gene

D) A mutation affecting the GAL4 gene that makes its activation domain nonfunctional

E) None of the above

Explanation / Answer

UDP-glucose-4-epimerase is responsible for the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism

homolog in galactosemia which is GALE implicated, can complement yeast null mutant, so mutation in this region is possible due to that further metablosm can not be posssible.

SO the answer shall be D means  A mutation affecting the GAL4 gene that makes its activation domain nonfunctional

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