HELP Match the following phenotypes with the mutation that would be the most lik

Question

HELP

Match the following phenotypes with the mutation that would be the most likely to be its cause.  The mutant proteins are listed below, followed by the protein domain that is mutated in each case, followed by the amino acid change that is a result of the mutation.  

Try to answer each question by using the following reasoning: if you observe a _________ phenotype, then that could be due to a mutation in protein _______, in which the ________ domain has had an amino acid change from ____ to ____.

Example:  

Q:  _____ After a few rounds of cell replication, yeast cells die and their chromosomes are much shorter than those found in wild-type yeast.

A:  telomerase (the most likely protein mutated to give you this phenotype)

_____  Mutant human cells die, and you observe that the DNA in the cells is not condensed prior to cell division

_____  E. coli DNA replication does not take place, and you observe no single-stranded DNA in the mutant cells

_____ In mutant E. coli, you observe Pol III core proteins floating freely in the cell, removed from the holoenzyme complex

_____ In mutant E. coli, you see very low processivity and you observe high disassociation of Pol III and the DNA strand

Protein            Domain                    Mutations

A)  Pol III            Thumb domain            Asp to Ala

B)  Pol III            Palm domain                Asp to Ala

C)  Pol III            Fingers domain            Asp to Ala

D)  SBP            DNA binding domain        Arg to Lys

E)  SBP            DNA binding domain        Arg to Pro

F)  Tao protein        Thumb domain            Thr to Ala       

G)  Tao protein    Thumb domain            Gly to Pro

H)  Pol             Thumb domain            Arg to Ala

I)  Cdt6             ORC-interacting domain         Glu to Asp

J)  Cdt6            ORC-interacting domain        Lys to Asp

K)  DNA gyrase     DNA-binding domain          Arg to Glu

L)  Rnase H        exonuclease domain        Phe to Ala

M)  DNA ligase        DNA binding domain        Ala to His

N)  Histone H1        DNA binding domain        Arg to Ala

O)  Histone H1        DNA binding domain        Arg to Lys

P)  Pol             Thumb domain            Thr to Ala

Q)  Pol             Palm domain                Arg to Asp

R)  DNA Helicase    ATPase domain            Met to Ala

S)  Sliding Clamp    Pol III-binding domain        Ala to Gln

T)  Telomerase        DNA binding domain        Arg to Glu

Explanation / Answer

_____ Mutant human cells die, and you observe that the DNA in the cells is not condensed prior to cell division

Ans: Histone H1 And Histone H1    (DNA binding domain ) ( Arg mutations) are going to cause a change in the binding of the DNA .

_____ E. coli DNA replication does not take place, and you observe no single-stranded DNA in the mutant cells

Ans: SBP, mutation in single stranded binding protein, will result in this mutation.

_____ In mutant E. coli, you observe Pol III core proteins floating freely in the cell, removed from the holoenzyme complex

Ans - pol III thumb domain , ( Asp to ala ) mutation. The thumb domain maintains interaction between the DNA polymerase and its substrate .

_____ In mutant E. coli, you see very low processivity and you observe high disassociation of Pol III and the DNA strand

Ans - sliding clamp mutation that is a pol III binding protein, when mutated would give this phenotype.

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