How would mutating the regulator protein allow you to differentiate between posi

Question

How would mutating the regulator protein allow you to differentiate between positive or negative control system? ( assume you're able to monitor change in gene expression levels)
Why not eliminate repressor/activators and just have corepressor/inducers act directly on DNA/RNA? How would mutating the regulator protein allow you to differentiate between positive or negative control system? ( assume you're able to monitor change in gene expression levels)
Why not eliminate repressor/activators and just have corepressor/inducers act directly on DNA/RNA?
Why not eliminate repressor/activators and just have corepressor/inducers act directly on DNA/RNA?

Explanation / Answer

In negative regulation a repressor protein from a regulator gene binds to an operator sequence to prevent a gene from being expressed. In positive regulation a transcription factor is required to bind at the promoter in order to enable RNA polymerase to initiate transcription.A site specific mutation that is brought in the regulatory gene in its operator or promoter binding nucleotide sequence results in production of mutant regulator protein that may fail to bind at regulatory sequence in DNA and fail to prevent RNA POLYMERASE binding at initiation sequence that leads to conversion of negative regulation to positive regulation

Repressor protein is a substance which acts on an operon to inhibit enzyme synthesis.is a substance that inhibits the expression of genes. ... A corepressor downregulates (or represses) the expression of genes by binding to and activating a repressor transcription factor.so corepressor can not used.A transcriptional activator is a protein (transcription factor) that increases gene transcription of a gene or set of genes. Most activators are DNA-binding proteins.

aninducer is a molecule that regulates gene expression. An inducercan bind to protein repressors or activators.Inducers function by disabling repressors. The gene is expressed because an inducer binds to the repressor.

Corepressor

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