The data shown below (figures A and B) were derived from a fluorescence recovery

Question

The data shown below (figures A and B) were derived from a fluorescence recovery after photobleaching (FRAP) study on a membrane protein found In red blood cells. Living cells were labeled with a fluorescent antibody that was specific for this protein. The panel labeled B reveals the Intensity of the antibody (blue fluorescence, F, or dark color) coming from the surface of the cells at different times (F_1, F_0, F_t) during the experiment. The trace shown in part A quantifies the fluorescence emission at these times. What does the pale area represent in F_0 and why is it disappearing in F_t?

Explanation / Answer

Florescence recovery after photobleaching (FRAP) is a technique for tracking the florescent protein molecule movement in an area upon bleaching by laser light. In the given graph (A) and diagram (B) Ft shows membrane proteins before photobleaching. All the proteins onto which antibodies bound are fluoresce. Upon treatment with light on the given area all the proteins got destroyed as a result of it there was no florescence Fo shown by pale area. After some time, membrane recover from bleaching and proteins present in the nearby area diffuse to the bleached area which filled the pale area.

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.