Answer the following questions with how the results of the 2 methods differ. a.

Question

Answer the following questions with how the results of the 2 methods differ.

a. What information would you get from a northern blot that you would not get from RT-(reverse transcriptase)PCR if used to detect the same mRNA?

b. What information would you get from a Southern blot that you would not get from a PCR experiment if used to detect the same genetic locus in human genomic DNA?

c. Given your answers to A and B, why is it more common to use the PCR based methods instead of the blot based methods?

Explanation / Answer

a) http://pathmicro.med.sc.edu/pcr/realtime-home.htm
b) http://www.ncbi.nlm.nih.gov/books/NBK7571/
c) http://www.scientific.org/tutorials/articles/riley/riley.html

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.