ammonia, which turns the indicator fuchsia. Tube (b) is the negdllVl FIGURE 15.5
ID: 164737 • Letter: A
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ammonia, which turns the indicator fuchsia. Tube (b) is the negdllVl FIGURE 15.5 Urease control. PROCEDURE First Period Label one tube of each medium with your assigned culture. Gelatin Hydrolysis Is it solid or liquid? 1. Examine the nutrient gelatin: What is the temperature of the laboratory? to do to If the gelatin is solid, what would you need liquefy it? To resolid it? c 2. Inoculate each tube with one of your assigned tures, using your inoculating needle. 3. Incubate the tubes at room temperature. Litmus Milk 1. Describe the appearance of litmus milk 2. Inoculate one tube with Pseudom omas BSL-2 and one tube with one of the other organisms. 3. Incubate the litmus milk at 35°C for 24 to 48 hours. Urease Test 1. Inoculate each urea agar slant with one of your as- signed cultures, using your inoculating loop. 2. Incubate the tubes for 24 to 48 hours at 35°C.Explanation / Answer
1. Gelatin is a semi solid colorless substance which becomes jelly when cooled and liquid on heating.
Gelatin is liquified by heating it. It normally liquefies at 28°C and above.
It is solidified by lowering the temperature, soaking it in cold water or by placing in the refrigirator
2)Litmus Milk is a differential media containing milk, litmus which is a pH and oxidation-reduction indicator, lactose, and casein (a milk protein). The components of litmus milk are metabolised by bacteria.It is used to differentiate and identify Lactic acid bacteria, Enterobacteria and Clostridium
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