Article: Figure 4a: Question: and binding site methylation specifically within a

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Article: Figure 4a: Question: and binding site methylation specifically within acute promydocytic leukaemia cells (NB), andboth interact with promyelokyticleukaemia (PML bodies" a sub nudear structure disrupted in PML cells. The anomalous behaviour ofthese two transcription factors with respect to chromatin structure and DNA methylation may thus be related to a specialized mechanism seen only in pathologically altered cells. A map of distal DHS-to-promoter connections From examination of DNasel profiles across many cell types observed that many known cell-selective enhancers become DHSs nchronously with the appearance of hypersensitivity at the pro- moter of their targetgene (supplementary Fig 13. To generalize this, we analysed the patterning of1A54,901 distal DHSS (DHSs separated from a Tss by at least one other DHS across 79 diverse cell types Distal DHSs conneenad Promoter DHSe connected (Supplementary Methods and Sapplementary Table 6), and corre- per promoter DHS per distal DHS lated the cross-cell type DNase signal at each DHS position with that at all promoters within 500 kb (Supplementary Rig, 14a). We 1-10 identified total of578905DHSethat were highly correlated (r 07 with at least one promoter (P

Explanation / Answer

DNA methylation decreases chromatin accessibility in over 95% cases. Increased methylation is negatively correlated in most cases with chromatin accessibility. In the Dnase I hypersensitive site the transcription factor levels and methylation at target site shows negative relation in all most 70% of the cases. It is also confirmed that the target site for methylation is carried out only after the removal of the transcription factor from that particular site. In the graph it shows some of the factors are positively regulated for methylation and transcription and in some others it shows negative regulation. Positive regulation means high methylation : high transcription ( high chromatin accessibility) Negative regulation means high methylation : low transacription.(low chromatin accessibility)

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