The instructions are on the first page. I already filled out the boxes. I need h

Question

The instructions are on the first page. I already filled out the boxes. I need help making the Dichotomous Key. Please and thank you BIOLOGY 2192, Homework #4a NAME A fellow student has produced a set of materials that a series of media all with the same unknown Gram negative organism, but she is unsure about how to interpret the results. Because of your kind nature, you have offered to help 1. For each test listed below, explain the observed results, describing your e metabolic end and indicators involved in each test,as well as products about the metabolism of the unknown or 2. 2192Gram and create process in dichotomous key using only the tests listed below, then highlight the decision your key to identify the unknown bacteria. Proteus Abbreviations: atrobacter freundli Escherichia coli EC, Klebsiela pneumoniae KP, vulgaris-P, Pseudomonas aeruginosa -PA, Serratia marcescens SM, Shigella flexneri SF you find the tubes. After pladng the required reagents into the tubes you observe that the MR tube appears red in color and the VP tube has no red ring near the surface Substrate(s) enzyme(s), and metabolic end product(s): Substrates. organic acids Indicator(s) and expected reaction(s) and with metabolic end product(s): ndicotur s s Muth red and Re NPA and B Rad Positive Jyllow go hive positive. Results interpretation for unknown organism (presence/absence of enzymes): results show that ond is tive sor a cohol waste (VE) Second you find the sucrose Durham tube. The media is yellow with a small bubble in the Durham tube Substrate(s), enzyme(s), and metabolic end product(s): Substrat e. sucrose, ducts. ore on acids Indicator(s) and expected reaction(s) and with metabolic end product(s): nor low positive for s gor Germentation acid wo te 2s elo of Co Results interpretation for unknown organism (presence/absence of enzymes): resu show that it is positive Por s acid woo she

Explanation / Answer

based on the observed results and dichotomous key the most probable organism should be Shigella flexneri BSL 2,because it is MR+ VP-,non lactse fermentating and forms clear colonies on Mac Conkey agar.On TSI agr it shows K/A, which means it only ferments glucose.

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