2) Consider the first reaction of glycolysis if it was not coupled to ATP hydrol

Question

2) Consider the first reaction of glycolysis if it was not coupled to ATP hydrolysis: Glucose Pi G6P H20 hydrolysis), what would you of glycolysis and ATP estimate to be the AG for the above reaction? bo Based on steady-state Concentrations of Glycolytic Metabolites in Erythrocytes (table 18.2 from slides), estimate the actual AG in erythrocytes? c) Draw an energy Diag the uncatalyzed reaction as well as ii) for an enzyme-catalyzed version of the above reaction. Label products, reactants, AG, AG, and any other important values

Explanation / Answer

a) Delta G for ATP hydrolysis is -30.5kJ/mole

Delta G for coupled reaction i.e Glucose +ATP =Glucose-6-p +ADP is -16.7 kJ/mole

So, the delta G of the given reaction is -16.7 +30.5 = 13.8 kJ/mole

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