which proteins are localized to cytoplasm which proteins are localized in peroxi
ID: 163352 • Letter: W
Question
which proteins are localized to cytoplasm
which proteins are localized in peroxisome
You are trying to identify the peroxisome-targeting sequence in the thiolase enzyme in yeast. The thiolase enzyme normally resides in the peroxisome and therefore must contain amino acid sequences that are used to target the enzyme for import into the peroxisome. To identify the targeting sequences, you create a set of hybrid genes that encode fusion proteins containing part of the thiolase protein fused to another protein, histidinol dehydrogenase (HDH). HDH is a cytosolic enzyme required for the synthesis of the amino acid histidine and cannot function if it is localized in the peroxisome. You genetically engineer a series of yeast cells to express these fusion proteins instead of their own versions of these enzymes. If the fusion proteins are imported into the peroxisome, the HDH portion of the protein cannot function and the yeast cells cannot grow on a medium lacking histidine. You obtain the results shown in the figure on the right.Explanation / Answer
Protein 4th and 5th are localized in the cytoplasm. It is mentioned in the question that when HDH is localized in peroxisome then in that case cell cannot grow in medium without histidine. In the case of 4th and 5th construct yeast cells are able to grow because cells were able to grow without histidine in the medium.
Protein 1st 2nd and 3rd will be localized in peroxisome as yeast cell is not able to grow when HDH is localized in peroxisomes
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.