The graduate student tested NK “activity” from 7 different spleens in the presen
ID: 163023 • Letter: T
Question
The graduate student tested NK “activity” from 7 different spleens in the presence of 2 toxins. They told you they both are toxic to NK cells based on looking at the graph he got from the data. You take the data to a statistician and they give you the information in the tables below. Do you agree with the graduate student? Explain & Justify your answer.
t-Test: Paired Two Sample for Means
t-Test: Paired Two Sample for Means
Media
Toxin A
Media
Toxin B
Mean
49.5
25.5
Mean
49.5
26
Variance
413.9
138.7
Variance
413.9
20.4
Observations
6
6
Observations
6
6
Pearson Correlation
-0.39107
Pearson Correlation
0.75526
Hypothesized Mean Difference
0
Hypothesized Mean Difference
0
df
5
df
5
t Stat
2.161081
t Stat
3.348611
P(T<=t) two-tail
0.083073
P(T<=t) two-tail
0.02036
t Critical two-tail
2.570582
t Critical two-tail
2.570582
This data only demonstrated a single “E:T ratio”. What does the “E” stand for and what could be the source of the “E”?
t-Test: Paired Two Sample for Means
t-Test: Paired Two Sample for Means
Media
Toxin A
Media
Toxin B
Mean
49.5
25.5
Mean
49.5
26
Variance
413.9
138.7
Variance
413.9
20.4
Observations
6
6
Observations
6
6
Pearson Correlation
-0.39107
Pearson Correlation
0.75526
Hypothesized Mean Difference
0
Hypothesized Mean Difference
0
df
5
df
5
t Stat
2.161081
t Stat
3.348611
P(T<=t) two-tail
0.083073
P(T<=t) two-tail
0.02036
t Critical two-tail
2.570582
t Critical two-tail
2.570582
Killing of YAC-1 cells by Mouse Splenocytes (100:1 E:T ratio) 60 50 40 30 20 10 Media Toxin A Toxin BExplanation / Answer
Ans. The E:T ratio means Effector to target cell ration. So, the E stands for Effector cell.
The experiment is carried out to see the efficacy of immune cells (say, NK cells, macrophages, B-cells, etc.) to kill target cells (the cells bearing specific target antigens or stimulated to be so).
The effector cells can be isolated from bone marrow or blood. These can also be used from B cell-line culture, too.
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