I need an answer with full details and step by step explanation Thanks 2. Some b

Question

I need an answer with full details and step by step explanation Thanks 2. Some bacteria make an enzyme called B-galactosidase that catalyzes the breakdown of the polysaccharide B-galactoside into monomers of galactose. a. What type of linkage is being broken in this reaction? b. Do you think this is an endergonic or exergonic reaction and why? c. Some bacteria were found with an amino acid mutation within the active site of B-galactosidase protein, and these bacteria were not able to breakdown B- galactoside polymers. Explain how the mutation could have affected enzyme function.

Explanation / Answer

Answer:

2)

a) Beta Glactosidase is the enzyme that break the bond between the disaccharides (Lactose) into monosaccharides . -1,4-glycosidic linkage is broken down by this enzyme between glucose and galactose.

b) It is a exergonic reaction. As energy is released during breakdown of bonds in disaccharide molecule. It is a catabolic reaction.

3)

Mutation could change the aminoacid sequence of the enzyme present in the active site. If aminoacid is muttated at active site, the substrate can bind to active site but breakdown of substrate (disaccharides) to form product is not observed. Enzyme are specific for substrate due to its specificity. So activity of the beta galactosidase is lost or reduced.

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