To study the mechanism of SERCA, you prepare membrane vesicles containing this p

Question

To study the mechanism of SERCA, you prepare membrane vesicles containing this protein oriented such that its ATP binding site is on the outer surface of the vesicle. To measure pump activity, you use an assay that detects the formation of inorganic phosphate in the medium When you add calcium and ATP to the medium, you observe phosphate production for only a short period of time. Only after the addition of calcimycin, a molecule that makes membranes selectively permeable to calcium, do you observe sustained phosphate production. Explain.

Explanation / Answer

the serco endoplasmic reticulum Ca 2+ ATPase and the plasma membrane Ca2+ ATPase have the highest affinity forcalcium removal from the cytoplasm and there is phosphate accumulation.

P type ATPase form phosphoprotein intermediate and go conformational changes during ATP hydrolysis. the E1P intermediate can phosphorylate ADP and E2P react with water.

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