The following figure shows part of a bacterial chromosome and the loci for arabi

Question

The following figure shows part of a bacterial chromosome and the loci for arabinose utilization (ara), leucine biosynthesis (leu), and resistance to streptomycin (str). Explain how you would determine the distances between the three loci by PI co-transduction (i.e. explain in detail how you would carry out the experiment) and the results you would get if ara and leu 60,000 bp apart and leu and str are 35,000 bp apart. You are given bacteriophage P1, a wild type bacterium (ara^+, leu^+, str^s) and a mutant bacterium for all three loci (ara^-, leu^-, str^R).

Explanation / Answer

Co transduction is a method of adding two genetic markers in one bacteriophage to transfer in new host bacteria. P1 type of bacteriophage shows generalized type of transduction in which a phage can carry out from its any part.

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