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11A. Which of the following proteins are likely to act as gene activators? (a) M

ID: 161930 • Letter: 1

Question

11A. Which of the following proteins are likely to act as gene activators?

(a)        MetA only

(b)       MetB only

(c)        MetC only

(d)       Both MetA and MetC

11B.    Which of the following proteins are likely to act as gene repressors?

(a)        MetA only

(b)       MetB only

(c)        MetC only

(d)       Both MetA and MetC

11C.    Which transcription factors are normally bound to the Psf promoter in the presence of Mg2+ only?

(a)        none

(b)       MetA only

(c)        MetA and Met B

(d)       MetA, MetB, and MetC

11D.    Which transcription factors are normally bound to the Psf promoter in the presence of both Mg2+ and Ca2+?

(a)        MetA and MetB

(b)       MetB and MetC

(c)        MetA and MetC

(d)       MetA, MetB, and MetC

You are interested in examining the Psf gene. It is known that is normally produced when cells are exposed to high levels of both calcium (Ca2 and magnesium (Mg2 MetA, MetB, and Meto are important for binding to the promoter of the Pst gene and are involved in regulating its transcription MetA binds to the "A" site in the promoter region, MetB to the "B" site, and MetC to the "C" site. You create binding-site mutations in the A, B, and C sites and observe what happens to transcription of the Pst gene. Your results are summarized in Table Q8-17 Transcription in the presence of Ca 2+ and/or Mg 2+ No Ca 2+ 2+ With Ca With With 2+ 2+ 2+ 2+ and Mg Binding sites present or Mg Ca Mg A, B, and C none A only B only C only A and B only A and C only B and C only For this table no transcription of Psf low level of transcription of Psf, and high levels of transcription of Psf. I Table Q8-17

Explanation / Answer

11)A)d)Both MetA and MetC because they both have + sign showing activations.leading to ++

11)B)(b)MetB only as it doesnt show any activation with a + sign.

11)C)a)None because the answer is only MET C

12)D)(d) MetA, MetB, and MetC as all of them are under the influence of both calcium and magnesium ions here.