Jill wants to isolate ribosomes from the cells she isresearching. She looks over

Question

Jill wants to isolate ribosomes from the cells she isresearching. She looks over figure 6.5 in her text and decidesto collect the tissue cells, homogenize them in the blender, andspin them at 150,000 g (150k times the force of gravity) for 3hours. After 3 hours, Jill sees a large pellet at the bottomof the tube, but soon realizes something is not right. What isthe problem? LIFE SAVER RATING!!! Jill wants to isolate ribosomes from the cells she isresearching. She looks over figure 6.5 in her text and decidesto collect the tissue cells, homogenize them in the blender, andspin them at 150,000 g (150k times the force of gravity) for 3hours. After 3 hours, Jill sees a large pellet at the bottomof the tube, but soon realizes something is not right. What isthe problem? LIFE SAVER RATING!!! LIFE SAVER RATING!!!

Explanation / Answer

Ribosomes   & other  cellular   organelles   are  separated    by   the   process   of Ultracentrifugation, which   has a  maximum    angular   velocity    of   about   70000 g . But   the   process   Jill    has  taken   is   similar to  Microcentrifugation   or  Superspeed   centrifugation   which    is   used  for   the   isolation  of   the   cell   &   the nuclei . In the   former process   the  maximum   angular   velocity  reaches   upto 130000 g  which   is   near    to    the    value   given    in   the   question . While   in the  superspeed   process   it  is   30000g.

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