a. Gene expression profiling was conducted to find genes associated with Autism

Question

a. Gene expression profiling was conducted to find genes associated with Autism Spectrum Disorder (ASD). This profile is showing the top 530 scoring genes. The control group is Universal Human Standard (bought from a biotech company) and the other 3 groups are sorted according to ASD phenotype. There are distinctive patterns for each ASD phenotype. What must be isolated from each patient in order to do this type of profiling? Once you have this molecule, what is the next step? Each row in this array represents a gene. If we look at a gene in this array, what are we learning about its expression?

Explanation / Answer

In order to do gene expression profiling and then to find the gene associated with ASD, First isolate the mRNA from both patient and control group. After getting the mRNA, synthesis the cDNA by using poly dTTT oligo and label both the sample (patient and control) with differential dye (like patient sample with cy3 and control sample with cy5). Then hybridize both the sample with microarray chip which is already having gene probe. and look for the expression pattern by analyzing the florescence signal. Most of the signal will be yellow but if you find any red or green signal that means some gene expression is going up or going down.

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