USI UR2: (15 Points) Match the following: 1. Gel Filtration l chromatography A.

Question

USI UR2: (15 Points) Match the following: 1. Gel Filtration l chromatography A. A plot of phi and psi angles 2. Ion exchange chroma 3. Zwitterions B. Has no free a-amino group proteins based on size 4. Nucleoside To which a helices, sheets, and turns Another name for dipolar molecules. differences Responsible for primary structure of proteins D. 5. Hydrophobic 6. Palindromes F. Used to separate proteins based on charge Ramachandran --G. Unusual base pairing 8. Cysteine 9. Secondary structuresA nitrogen containing an aromatic base with a H. ribose 10. Proline 11. Peptide bonds 12. domains J. Retains their function even if separated from the proteir K. These sequences have two-fold symmetry L. Results in formation of a abasic site M. The effect that stabilizes the structure of DNA due to base stacking. N. Reducing agent O. An Amino acid required for disulfide bond 14. Depurination 15. DTT formation Answers: (No need to write the whole sentences, just A, B, etc.) 6. 12. 7. 2 13 8. 3. 4. 5. 14. 15. 10. 10

Explanation / Answer

1. Gel filtration Chromatography --> C

Reason: Gel filtration is a non-binding methodand simple to use and allow separation of proteins with differences in molecular size.

2. Ion exchange Chromatography --> F

Reason: It helps for the separation of ionizable moleculesbased on their total charge.

3. Zwitterons --> E

Reason: A molecule or ion having two or more functional groups of which at least one has a positive charge and the other has a negative charge.

4. Nucleoside --> I

Reason: A nucleoside consists of a nitrogenous base covalently attached to sugar but without a phosphide group.

5. Hydrophobic --> M

Reason: The structure of the DNA helix is stabilized by van der Waals forces, hydrogen bonds between complementary organic bases, and hydrophobic interactions between the nitrogenous bases and the surrounding water.

6. Palindromes --> K

Reason: These sequences has two fold symmetry or complementary symmetry

7. Ramachandran --> G

Reason: Phi and Psi are torsion angles used in computer models developed by Ramachandran for small polypeptides.

8. Cysteine --> O

Reason: Amino acid containing Sulfur. The disulphide bonds in proteins are formed between Thiol groups of Cysteine residues by the process of Oxidative folding.

9. Secondary structures --> D

Reason: Protein structures within long protein chains have regions in which chains are organized into regular structure known as alpha helices, beta sheets and turns.

10. Proline --> B

Reason: Proline is a peptide bond and it does not have a hydrogen in alpha-amino group

11. Peptide bonds --> H

Reason: It is responsible for particular function contributing to the overall role in protein.

12. Domains --> J

Reason: It rest of the proteins are destroyed, a domain still have its function

13. Hoogsteen --> G

Reason: The nucleotide base (A) is flipped resulting in unusual base pairing

14. Depurination --> L

Reason: An abasic site is created in DNA when glycosidic bond connecting a purine base to the deoxyribose sugar is cleaved.

15. DTT --> N

Reason: DTT is called Dithiothreital. It is used as a reducing agent for theodated DNA.

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