\\ Question Completion Status: All of the above QUESTION 5 2 points Save Answer

Question

Question Completion Status: All of the above QUESTION 5 2 points Save Answer You have purified a multisubunit extracellular protein that has several interchairn disulfide bonds. Which of the following chemicals would you add to your purified protein mixture if you wanted to eliminate the disulfide bonds? ODTT, a reducing agent O NaCI, a salt O H202, an oxidizing agent O SDS, an ionic detergent and denaturing agent O Tris, a buffering agent QUESTION 6 2 pointsSave Answer An enzyme acts on a tyrosine residue in a target protein to create a binding site for

Explanation / Answer

(d) SDS

Sodium docedyl sulphate.

and DTT.

Both will work.

It will reduce the disulphide S-S bonds to by addition of protons to

-SH bonds

Related Questions

Navigate

• Browse (All)
• Browse #
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.