An experiment is designed to study the mechanism of sucrose uptakeby plant cells

Question

An experiment is designed to study the mechanism of sucrose uptakeby plant cells. Cells are
immersed in a sucrose solution, and the pH of the surroundingsolution is monitored with a pH
meter. The measurements show that sucrose uptake by the plant cellsraises the pH of the
surrounding solution. The magnitude of the pH change isproportional to the starting
concentration of sucrose in the extracellular solution. A metabolicpoison that blocks the ability
of the cells to regenerate ATP also inhibits the pH change in thesurrounding solution. Explain
the results.

thanks

Explanation / Answer

ATP synthase uses protons moving down gradient in order to form ATPfrom ADP. Use of proton gradient consumes H+ raisingpH. When enzyme is shut down, consumption of H+ stops and pHremains low. ADP + Pi --->   ATP + HOH

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