Which of the following sets of primers could you use to amplify the target DNA s
ID: 15469 • Letter: W
Question
Which of the following sets of primers could you use to amplify the target DNA sequence below, which is part of the last protein-coding exon of the gene involved in cystic fibrosis? … represents the portion of the gene that is approximately 1,000 bp. Show the positions of all eight primers and explain why they can or cannot be used for amplification.5’- ggctaagatctgaattttccgag … ttgggcaataatgtagcgcctt - 3’
3’- ccgattctagacttaaaaggctc … aacccgttattacatcgcggaa – 5’
a) 5’ GGAAAATTCAGATCTTAG 3’;
5’ TGGGCAATAATGTAGCGC 3’
b) 5’ GCTAAGATCTGAATTTTC 3’;
3’ ACCCGTTATTACATCGCG 5’
c) 3’ GATTCTAGACTTAAAAGGC 5’;
3’ ACCCGTTATTACATCGCG 5’
d) 5’ GCTAAGATCTGAATTTTC 3’;
5’ TGGGCAATAATGTAGCGC 3’.
Explanation / Answer
Which of the following sets of primers could you use to amplify the target DNA sequence below, which is part of the last protein-coding exon of the gene involved in cystic fibrosis? … represents the portion of the gene that is approximately 1,000 bp. Show the positions of all eight primers and explain why they can or cannot be used for amplification.
5’- ggctaagatctgaattttccgag … ttgggcaataatgtagcgcctt - 3’
3’- ccgattctagacttaaaaggctc … aacccgttattacatcgcggaa – 5’
a) 5’ GGAAAATTCAGATCTTAG 3’;
5’ TGGGCAATAATGTAGCGC 3’
b) 5’ GCTAAGATCTGAATTTTC 3’;
3’ ACCCGTTATTACATCGCG 5’
c) 3’ GATTCTAGACTTAAAAGGC 5’;
3’ ACCCGTTATTACATCGCG 5’
d) 5’ GCTAAGATCTGAATTTTC 3’;
5’ TGGGCAATAATGTAGCGC 3’.
sequence are complementary
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