Jane and Hemi had a solution containing four proteins and analysed it by SDS-PAG

Question

Jane and Hemi had a solution containing four proteins and analysed it by SDS-PAGE. The four proteins A, B, C and D had subunit molecular weights of 10 kDa, 20 kDa, 30 kDa and 15 kDa respectively, with the quaternary structures of the proteins being monomeric, tetrameric, dimer and tetrameric, respectively. Which would travel the furthest on the gel (in order of most distance to least distance)? Second least distanceose ' Most distance Second most distance Choose.. Least distance Choose...v Choose Choose.

Explanation / Answer

Second least distance: 15kDa

Most distance: 30KDa

Second most distance: 20kDa

Least distance: 10kDa

Separation of proteins based on their molecular weight. Different proteins in their native state with the same molecular weight would migrate at different speeds in an electrical field depending on their charge and 3D shape.When the proteins are running in the gel, they get separated based on their molecular weight (mw), where high mw protiens move more slowly through the porous acrylamide gel than lower molecular weight proteins. The size of the pores in the gel can be altered depending on the size of the proteins you want to separate by changing the acrylamide concentration.

Related Questions

Navigate

• Browse (All)
• Browse J
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.