You discover a new compound that yeast requires for survival. To find genes invo

Question

You discover a new compound that yeast requires for survival. To find genes involved in the synthesis of this compound (compound X) you have screened for mutants that cannot grow on minimal media, but can grow on minimal media + compound X. You find 10 mutants and perform complementation tests. The results are shown below (+ = growth, - = no growth). How many genes are in your mutant collection? 2. You discover a new compound that yeast require for survival. To find genes involved in the synthesis of this compound (compound X) you have screened for mutants that cannot grow on minimal media, but can grown on minimal media + compound X. You find 10 mutants and perform a complementation tests. The results are shown below (+growth,- no growth) 1 23 4 5 6 7 8 9 10 2 6 8 10 How many genes are in your mutant collection? Which mutations are in the same genes?

Explanation / Answer

Ans. In above result, there are a total of 10 mutants that show mutation in 10 different genes, which are involved in the synthesis of that X compound. It shows the complementation test that involves testing of complementation (generation of a wild type phenotype by two strains having recessive mutations in different genes).

The mutated genes include 4 and 1, 2 and 3, 2 and 7, 3 and 7, 5 and 6, 5 and 9, 6 and 9, and 8 and 10 that cannot complement each other.

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