You are measuring the effect of temperature on the rate of an enzyme-catalyzed r

Question

You are measuring the effect of temperature on the rate of an enzyme-catalyzed reaction. If you plot reaction rate against temperature, which of the graphs below would you expect your plot to resemble? Explain why temperature has this effect. 9. 2. 1. 100 100 3. 100 100 Temperature C) 10. You have a purified antibody. You treat the antibody either with or without DTT (a chemical that breaks disulfide bonds). You run a protein electrophoresis gel and find that the sample treated with DTT has two bands, one at 25 kDa and one at 50 kDa. However, the untreated sample (no DTT) has only one band at 75 kDa. Explain the reason for the different band sizes

Explanation / Answer

9--- ist graph is of right type.

It is found that at every 10 degree rise in temperature, enzyme efficiency increase from 50% to 100% and reaction rate is increased as seen in graph . After attaining optimum temperature i.e. 40 degree celsius for our body enzyme, enzyme efficiency decrease due the denaturation of enzymatic proteineaceous part(apozyme) and reaction rate decrease.

10-- after treating antibody with DTT , the disulphide bond between light chain and heavy chain break down due to which whwn sample run on gel electrophoresis two bands are found one of high chain(50KDa) and other of light chain(25KDa)

without treating with DTT: antibody maintains its disulphide bond and we get only single band of 75KDa in gel electriphoresis.

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