9. M spectrophotometer easure the absorbance of each tube at 595 nm. The tubes f
ID: 151462 • Letter: 9
Question
9. M spectrophotometer easure the absorbance of each tube at 595 nm. The tubes fit directly into the sample holder of the Enter your raw data for the absorbance measurements of the samples from the bovine serum albumin (BSA) standard curve on the table below. Tube volume BSA (HL) Asss Mean Ases g of protein 0.05 . 1.5 O.19 .IO 10 10 15 15 20 O.23 .33 o.30 0.54 o 43 O.48 0.47 o.52 o.50 o.60 10 30 40 40 12 13 14 15 16 17 Calculate the average absorbance value for each of the duplicate samples. Determine the total amount of protein in each of the pairs of tubes. Remember that the stock solution is 1.0 mg/mL or 1.0 gJuL. Example: if tube 2 contains 5 L of 1.0 gJuL of BSA solution, then it contains a total of 5 g of protein. 10.Explanation / Answer
9.
Tube Volume BSA (ul) A595 Mean A595 ug of Protein 1 0 0 0 0 2 5 0.05 3 5 0.15 0.1 0.1 4 10 0.19 5 10 0.10 0.15 0.15 6 15 0.17 7 15 0.23 0.2 0.2 8 20 0.33 9 20 0.30 0.32 0.32 10 30 0.54 11 30 0.43 0.49 0.49 12 40 0.48 13 40 0.47 0.46 0.46 14 50 0.52 15 50 0.50 0.51 0.51 16 60 0.60 17 60 0.60 0.60 0.6Related Questions
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