Eight mutant bacteriophage strains cannot lyse a certain type of bacteria that c

Question

Eight mutant bacteriophage strains cannot lyse a certain type of bacteria that can be lysed by wild-type bacteriophages. The mutant strains were allowed to infect the bacteria in a complementation test. A "+" indicates that lysis occurred with coinfection. A "-" indicates that lysis did not occur.

A cistron is defined by no complementation in the _____ configuration.

How many genes are mutated in this experiment? (Use a number not a word in the space) ________

Which of the following strains below are defective in the same gene as strain 6? Answer yes if the strain is defective or no if it is not. Strain Defective

1)

2)

3)

4)

5)

6)

7)

8)

1 2 3 4 5 6 7 8 1 - + + + + + - + 2 - + - + - + + 3 - + - + + + 4 - + - + + 5 - + + + 6 - + + 7 - + 8 -

Explanation / Answer

Cistron is defined by functional( coding ) part of genetic material.So, it must get incorporated in cis configuration for doing effective transformation.

Hence,cistron will show no configuration in trans configuration.

Here, 4 genes are mutated

gene1 of bacteriophage( as it can't lyse 1;7 strain of bacteria)

gene 2 of bacteriophage( as it can't lyse 2, 4;6 strain of bacteria)

gene 3 of bacteriophage ( as it can't lyse 3; 5 strain of bacteria)

& gene 4 of bacteriophage( as it can't lyse 4; 6 strain of bacteria)

As the 6th strain is defective in the same manner, 5th & 7th strains are defective because they only can't lyse their own numbered strain of bacteria.

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