1o minute of solsing solucion to bring the toral amoune up to 10 ml t in dhe ke
ID: 151103 • Letter: 1
Question
1o minute of solsing solucion to bring the toral amoune up to 10 ml t in dhe ke uneld ir's eadly bo be used by the swlenns and waser to each of the4ese tubses wich the amoune designated on Table 3.3 s. Thm al a5 ml of the chloruplase musture that your TA will sive you to test tubes 1, Z, and 4 test tobes with cape with the eest tube number and your group number buffer Seudens I Add the 05 ml of DCFIP your last step into test tubes 2, 3, and Oap all 4of the nest tues Whap rube d completely with aluinum foil 7. Mis the contents of each tube well . Recond the current colors of each test rube in Table 3.4 under "Before Observed Result . Place she 4 sest tubes in front of the light. You should have someone keep an eye on them as the reaction will occur within minutes (approximately 3-5 misutes). 10. Record expectations for each tube in Table 3.4 while you wait. I1. Afser 5 minutes, recond the "Afer Ohserved Results" in Table 3.4 12. Dapose all solutions from the 4 rese tubes in the bottle labeled "DCPIP Waste" in the fume hood. 02 mm DCPIP 05ml 2 mal 2 nl 2 wl. 2 mL 0.5 L 0.5 ml. 0.5 ml 0.5 ml 5 ml. I ml DCPIP RESULTS Tube # Expected Revults Before Observed Results After Observed Results leolor) Explanation Be 3-8 EXERCISE 3Explanation / Answer
Which of the four tubes serve as negative control for chloroplast and why?
Tube 3 will be the negative control as chloroplast is not added in this tube.
Explanation: In experiments the negative control is the group which must not give results. The purpose of introduction of negative control is to eliminate chances of false positive. Contrary to this, a positive control is the group which must be give results. The purpose of this group is group is to remove chances of false negative.
Which of the four tubes serve as negative control for DCPIP and why?
Tube 1 will be the negative control as DCPIP is not added in this tube.
Which of the four tubes serve as positive control and why?
Tube 2 will be the positive control as it contains both chloroplast and CPIP as well as it exposed to light for photosynthesis.
What is the purpose of covering tube six with foil?
Tube six is cover with foil to inhibit entry of sunlight into tube to inhibit photosynthesis.
Why was there some color change in tube 4 which was wrapped in aluminum foil?
Some reactions in photosynthesis are not requires light called as dark reactions (Calvin Benison Cycle). The color change is observed due to those reactions.
Is there an electron being accepted in tube 1 even through there is no DCPIP present? Explain your answer.
Yes, the electrons in tube 1 is accepted by the electron transport chain of chloroplast. DCPIP has a higher affinity for electrons than ferredoxin and the photosynthetic electron transport chain can reduce DCPIP as a substitute for NADP+ and forms a colorless substance. If DCPIP is absent in system, the NADP+ occur as final electron carrier in photosynthesis.
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