2018_P54-1.pdf - Adobe Reader File Edit View Window Help 153% Tools Fill & Sign

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2018_P54-1.pdf - Adobe Reader File Edit View Window Help 153% Tools Fill & Sign Comment A) Given that the lowest energy stretching for CO (from the above diagram has a wavenumber of 2652 cm1: 1. Calculate the wavelength (in nm) for this stretching frequency 2. Calculate the energy of this stretching frequency 1.5. Two oxygen atoms (rdw-1.4 A) are separated by a distance of 2.9 A. The depth of the potential energy well is 0.5 kJ/mol. Calculate the van der Waals energy for the two oxygen atoms. 1.6. The following table shows the mean residue ellipticity measured for an unknown protein, that you have determined through other means contains 120 amino acids, that is in a helix-coil transition Unknown -Helix Ol 210 nm -4.4 195 nm 20 210 nm -8 195 nm 195 nm 210 nm A) Calculate the percent of -helix and coil in this protein B) Determine the number of amino acids in both the helix and coil confirmations C) At the midpoint on the thermal melting curve, determine the -helix and coil percent PChem Journal of the Week The PChem Journal should act as a way to reflect upon what you are learning and provide a space for you to determine what you are strong at, weak at, and excited for in the subject te at least one sentence on the followina torn 12:12 AM 9/27/2018

Explanation / Answer

Answer A

Formula for calculating % of helix

= (MRE – 2340/30300) X 100

=7.7%

% of random coil at rest 92.3%

Answer B

Number of total amino acid =120

No. of amino acid in alpha helix conformation = (7.7/100) X 120

= 9.24

Number of amino acid in coil conformation = (92.3/100) X 120

=110.76

Answer C

Denaturation midpoint has both folded and unfolded states at equilibrium therefore the protein has 50%helix & 50% coil

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