automated broth microdilution using VITEK, automated broth microdilution using M
ID: 150935 • Letter: A
Question
automated broth microdilution using VITEK, automated broth microdilution using Microscan (prompt method), agar/tube microdilution, e-test, or Kirby Bauer disk diffusion.
1) Choose a method and, using the advantages and limitations of the selected method as well as the advantages and limitations of the other options, justify your selection.
She also asks if you would want qualitative or quantitative susceptibilities displayed to providers.
2) Provide a recommendation regarding the display of quantitative or qualitative susceptibilities. (Note: your answer should explain advantages and limitations and providing quantitative susceptibilities to end users)
Explanation / Answer
Answer
VITEK test is used for the automated quantitative or qualitative susceptibility testing of isolated colonies for the most clinically significant aerobic gram-negative bacilli.
Advantages of VITEK test
species identification and antimicrobial susceptibility testing are speed and better workflow.
The reporting time for CoNS identification by the VITEK system ranged from 4.25h to 8h,
the mean time to result was 5.79h.
MicroScan method is mostly used to report values higher than broth microdilution method and this can lead to use of alternative agents.
Disk diffusion test, or Agar diffusion test, or Kirby–Bauer test used to test the antibiotic sensitivity in bacteria & uses antibiotic disc to test. In this test, the wafers containing antibiotics are placed on an agar plate where bacteria have been placed, and the plate is left to incubate. If an antibiotic stops the bacteria from killing or growing the bacteria, there will be an area around the wafer where the bacteria have not grown enough to be visible.
Advantages are to allow one to determine the most effective antibiotic to treat a given infectious disease.
Disadvantage is that it takes some time (approximate of 24 hours) to run and it doesn't indicate the dose necessary to kill or inhibit the pathogen long enough for the defense mechanisms of the person to control the infection.
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