me nsert Calbri (Body)11 A-A A EI ! M.abe x2 x2 A, p . . Styles te 5. How will y

Question

me nsert Calbri (Body)11 A-A A EI ! M.abe x2 x2 A, p . . Styles te 5. How will you test for a successful cell modification? Using Protoplast Fusion to Create "Spiderman" Protoplast Cultures fuse together two cells that have no cell wall. Scientists use various mechanical (micropipette), electrical (electrofusion) or chemical (polyethylene glycol) means to encourage the cells to form a hybrid. Because these methods are random and uncontrolled, the protoplasts rarely form a true hybrid. However, if (when) a hybrid is formed, it may regenerate a cell wall, grow, and divide. This hybrid culture can expand using special media. This technique is used mostly for plants, fungi (yeast) and plant.fungi hybrids. It has been used to improve the production of cephalosporin C and increase plant resistance to certain viruses How might you select for the hybrid you want? What is the difference between intraspecific protoplast fusion and interspecific protoplast fusion? You work in a lab that is not restricted by money, NIH or ethical concerns. You have a lot of time on your hands, so you decide to create an interspecific protoplast cell culture between a man and a spider. You want to study the resulting hybrid to determine if a superhero could really exist. Using what you have learned about protoplast fusion, come up with your protocol. (Include: specific cells used, protoplast method, hybrid selection, culturing concerns, future plans). 1. 2. 3. References Wikipedia. Ti Plasmid. May 2013. (Retrieved July 2015). https://en wikipedia.org/wiki/Ti plasmid Page 2 o1 2 42

Explanation / Answer

The protoplast culture for inter-cellular hybrid between human and spider can be found as below:

Thus, these are the steps which can be performed to generate a hybrid between human and spider cells to understand the features of the hybrid.

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