7. Prokaryotes and eukaryotes carry out transcription using very similar machine

Question

7. Prokaryotes and eukaryotes carry out transcription using very similar machinery, but there are also distinct differences. 7a. Circle the components below that are present in eukaryotes but NOT in bacteria. [3 pts) RNA Polymerase Promoter Enhancer Splicing 5' Cap Poly (A) Tail Mediator TATA box Terminator Sigma Factor 7b. When a region of DNA that contains the genetic information for a protein is isolated from a bacterial cell and inserted into a eukaryotic cell in a proper position between a promoter and a terminator, the resulting cell usually produces the correct protein. But when the experiment is done in the reverse direction (eukaryotic DNA into a bacterial cell), the correct protein is often not produced even though transcription is carried out. The absence of which of the component(s) in 7a in bacteria may explain this observation? [4 pts] AMS

Explanation / Answer

7a.  

Splicing , 5`cap, polyA-tail, TATA box,  

7b.

Splicing is the main factor which is absent in prokaryotes, so there does not occur intron removing. Niether there is post- transcriptional modification like 5`capping nor polyA-tailing. Also there is no proper post-translational modifications in the prokaryotic system for eukaryotic protiens.

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