Instructor: Cabrera B 1105 Homework \"2 (based on C. 2 and 3) 4b. What type of b

Question

Instructor: Cabrera B 1105 Homework "2 (based on C. 2 and 3) 4b. What type of bond would hold two such adjacent nucleotides together in a growing nucleic acid chain? In the diagram, circle the groupls) that would participate in the formation of this bond if the nucleotide shown above were added to the growing nucleis acikd chain. 13 pts 4c. Name the type of bond that the above nucleotide will form with its complementary nucleotide. How many of these bonds would you expect between this nucleotide pair? (Hint: to answer this question you must first ldentify the nitrogenous base in the pictured nucleotide.) [3 pts) e student wants to perform an in vitro (in a test tube) transcription reaction to produce et up the reaction, he adds DNA templates with the gene of 5. A graduat mRNA from a gene he was studying. Tos interest, RNA polymerase, and ribonucleoside monophosphates (a mixture of CMP) to an Eppendorf added is not suitable for the reaction. Which component is she referring to? Explain your answer. [4 pts] tube. Before he proceeds, his colleague tells him that one of the components he 6a. The two strands of a DNA molecule can be separated by the application of heat and without breaking any covalent bonds. However, every unique DNA molecule "melts" at a different temperature. In this context, Tm (melting temperature) is the point at which two strands separate, or become denatured. Write out the order of the DNA sequences listed below according to relative melting temperatures (from lowest Tm to highest Tm). Assume that they all begin as stable double-stranded DNA molecules. [3 pts] A CTAACTGG B. TATTGTCT C. GACTCCTG D. GGCGCACC From lowest Tm to highest T

Explanation / Answer

5)she is referring to ribonucleotide monophosphates because in a transcription reaction triphosphates are required.

6) lowest to highest, B<A<C<D

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