calculate the. protein concentration misit ey inrpolatian in the ntarndar cuntra

Question

calculate the. protein concentration

misit ey inrpolatian in the ntarndar cuntratbion of msitiochndml Obtain a slution of protein wheoe concentration in known Label a set of tusd tubes (13 X TI mm) as B and 1 to S using Procedure Construct a table in your noecbook (ce example budow) Tube Img/ml0.15 M BSAGil.) / Naci (It.), reagent(mL) | (Ag/tube) 40 2.5 3 3020 20 343 4 40 10 2.5 Bradfor Sample Tube N" Dilution Protein factorugltube) mitochondria M2 222100 022 -1000 1:100 104 M4 1:1000 wrGi WC2 WG3 103 wheat germ 1:10 10 2.5 O- BO Y 100 1:100 to1000 103 .Add 2.5 ml of the Bradford reagent to each tube. Wait at least 5 minutes and then measure A Select the Ass value that falls in the middle region of the standard curve. This is the sample that falls within the useful range of the standard curve. .

Explanation / Answer

Bradford protein assay is the traditional method for calculating protein concentration of unknown sample.

Performe the assay and calculate standarde. The result should be aroung 0.5mg/ml. To calculate the concentration of undiluted, unknown sample, simply multiply by the dilution factor. so 0.5x10=5ml

as the image of your question is not clear as per my understandinding

0.206x10=2.06

0.30x100=30microgram/tube

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