please explain your answer Thanks o 9/25/2018 11:55 PM3109/25/2018 07:17 PM D Gr
ID: 150169 • Letter: P
Question
please explain your answer
Thanks
o 9/25/2018 11:55 PM3109/25/2018 07:17 PM D Gradebook Print Question 16 of 39 The following t abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. table shows where different restriction endonucleases (restriction enzymes) cleave DNA. TMaPd Enzyme Target sequenceCleevege EcoR EcoRV 5 GATATC 3 Haelll Hindlll PpuMI5 RGGWCCY 3 5 GAATTC 3' 3 CTTAAG 5 5 G G 5 5 GAT ATC 3 3' CTA TAG 5 3 CTATAG 5 5 GGCC 3 3' CCGG 5. 'CC GG 5 5' AAGCTT 3 3. TTCGAA 5. 5' A 3. TTCGA AGCTT 3 A5. 5 RG GWCCY3 3. YCCWGGR 5' | 3. YCCWG GR 5 Which restriction endonuclease(s) would cleave a DNA molecule at the following sequences? a) 5 ATCGAACTAGGCC 3 EcoRV EcoR Haell Hindi; b) 5 TCCAAGCTTGAATTO 3 ECOR Hindill Hael ECORV below. Plasmid DNA and a gene of interest are out with the enzyme RpuMi Write a possible sequence of bases for the sticky end of the gene in the 5 to 3 di 5t 3" Hint PreviousExplanation / Answer
According to the given data the following answer is suitable:-
A) In the a sequence HaeIII enzyme cut the DNA strand because the specific sequence are present in the DNA. In the dna strand there are at last GGCC is present then at that sequence Hae III enzyme can cut and make a cut of GG/CC in 5'-3' sequence.
B) In the second sequence of DNA there are EcoRI enzyme can cut the DNA sequence because at last the sequence of that enzyme is present that's GAATTC and after cut it can make G/AATTC sequence.
2) In the PpuMI enzyme it can cut the sequence RGGWCCY and make cut at RG/ GWCCY in DNA. The sequence of DNA may be A/G GG A/T CC C/T
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.