Under which of the following conditions would wash out occur in continuous cultu

Question

Under which of the following conditions would wash out occur in continuous culture?

Select one:

a. The dilution rate exceeds the substrate level.

b. The substrate level does not exceed the requirements for maintenance energy.

c. The dilution rate exceeds the capability of the cells to grow.

d. Both the dilution rate exceeding the capability of the cells to grow and the substrate level not exceeding the requirements for maintenance energy.

e. Both the dilution rate exceeding the capability of the cells to grow, and the dilution rate exceeding the substrate level.

Explanation / Answer

Subculturing, also referred to as passaging, is the removal of the medium and transfer
of cells from a previous culture into fresh growth medium, a procedure that enables the
further propagation of the cell line or cell strain.
The growth of cells in culture proceeds from the lag phase following seeding to the log
phase, where the cells proliferate exponentially. When the cells in adherent cultures
occupy all the available substrate and have no room left for expansion, or when the cells
in suspension cultures exceed the capacity of the medium to support further growth, cell
proliferation is greatly reduced or ceases entirely. To keep them at
an optimal density for continued growth and to stimulate further proliferation, the culture
has to divided and fresh medium supplied.

Based on above criteria washing is needed when all substrate consume by cells and there was no space for cell to adhere on substrate.

Option D is correct.

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