Eco0109l 2674 Aat lI 2617 BstAPI 179 del) 183 Ehel 235 Sspl 2501 Xmnl 2294 Bcgl

Question

Eco0109l 2674 Aat lI 2617 BstAPI 179 del) 183 Ehel 235 Sspl 2501 Xmnl 2294 Bcgl 2215 Scal) 2177 2486 469 pUC18/19 2686 bp Sapl 683 852 AflI,BsplU1 11 806 Gsul 1784 1 626 Cfr10l 1779 1466 Eco31l 1766 rep (p Eam 1105l 1694 AwNl 1217 single cut because it's dust one What size band(s) would you predict if you digest with Scal? Assume the digest goes to completion cut 2686 What size band(s) would you predict if you digest with both Scal and Ndel? Assume the digest goes to ca75@2177 UNcuts/olasrnd at lg3 bp completion an distance bet 21-183: 1994 bp 2686-1994:692 bp Describe what bands you would see on the gel if the Scal/Ndel digest in part b does NOT go to completion Question 3 work space Seal Nolee are not you wll have undiges Super coile a procue

Explanation / Answer

The given plasmid is pUC18/19 of 2686basepairs.

In the first question,digestion of scal occurs,where single complete digestion,so the value is 2686,because it is one single cut.

In the second question,both scal and Ndel plasmids are removed,i.e two size bands scal is 2177 and Ndel cuts at 183 base pairs gives

2177-183=1994base pairs.

Distance between pUC to given base pairs is 2636-1994=692 base pairs

In the question three,it can be answered in the following way,apart from scal /Ndel digestion,there will some more single linear products obtained so,two products that can be predicted asAfl3,BspLU11.

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