You wish to separate a mixture of glutamic acid, lysine, and arginine amino acid

Question

You wish to separate a mixture of glutamic acid, lysine, and arginine amino acids by ion exchange chromatography. Which of the following conditions would be most appropriate to allow the glutamic acid to elute with the LONGEST retention time.

A. Add the mixture to a cation exchange column at pH 7.

B. Add the mixture to a cation exchange column at pH 3.

C. Add the mixture to an anion exchange column at pH 3.

D. Add the mixture to an uncharged stationary phase column at pH 7.

E. Add the mixture to an anion exchange column at pH 7

Can someone explain why the answer is E and how to go about this question

Explanation / Answer

Since the arg and lys are positive amino acid so they will not remain as the column is positive charge and will repel each other but on other hand glutamic acid negatively charge will bind to the column eluting the catonic amino acid first therefore the answer is E where anionic amino acid is exchange with the positive coloumn leading to anionic exchange chromotography.

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