1Label 7 the correct beaker (Note D1 water is 0.0M) 4 Place all seven potato sam

Question

1Label 7 the correct beaker (Note D1 water is 0.0M) 4 Place all seven potato samples ind place 5. Remove a cylin 6. Weigh it to neare petri dish, and keep them covered to prevent their drying out t between the folds of a p aluminam heet on the balance Record the weight in Table 1 in the , carefully recording the weight and placing the potato into the 0o Mand Repeat steps 5-6 for each potato piece correct beaker solution from 0.1 to 0.6 M. "Be sure that the initial I weight of the cylinder placed in each test solution is accurately recorded. 9, Swirl each beaker 10. At the end of the incu urs. (As this takes place, you will be performing other lab activities.) er every 10 to 15 miutes as the potato pieces incubate ation period, record the time when the potato pieces are removed. Record the approximate incubation time in Tuble 1 1 Remove the pi olution only potato pieces from the first sampie Blot the pieces on a paper towel, removing excess 2 Weigh the potato pieces and record the final weight in Table 1 13. Repeat this were initially placed in the procedure until all samples have been weighed in the chronological order in which they test solutions 14. Record your data in the Results section Table 1. Data for Experiment Estimating Osmolarity by Change in Weight Sucrose Molarity 0.0 0.1 1 0.2 1.0.3.10.40.5 1.96 Final weight (g) | Initial weight (g) Weight change (g) 400. o02 04-o.+ the value be negative. If the sample gained in weight, the value should be positive. If it lost in w Discussion 1. At what sucrose molarity does the curve cross the zero change line on the graph? at O. 2. Explain how this information can be used to determine the osmolarity of the potato tuber tissue 3. Estimate the osmolarity of the potato tuber tissue 206

Explanation / Answer

3) osmolarity of potato tuber 0.3M because at this molarity no change in weight occurs

2) the results of the experiment have shown that the osmolarity of the potato tuber is approximately 0.3 M because it did not gain nor lose weight in the 0.3 M sucrose solution; there was no net movement of water. at molarity 0.6M the change in weight is maximum which means 0.6M sucrose solution is hypertonic. Looking at this experiment osmosis had definitely taken place during incubation and gave results as predicted.

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