using the above figure, pls answer the bottom one 35 30 2 25 High % O2 320 E 15-

Question

using the above figure, pls answer the bottom one

35 30 2 25 High % O2 320 E 15- 10 Low % O2 0 0 2 3 4 Incubation period (days) Figure 4 The emergence of WS-like genotypes is affected by O2 conditions. Shown is the emergence of WS-like genotypes in static microcosms incubated under high (circles) and low O (squares) conditions. Microcosms were inoculated with 10' wild type SB w25 cells (i.e. 0% ws-like). Low Oz levels were produced using AnaeroGen Compact bags. High O, levels were provided by normal atmospheric conditions. Replicate microcosms were destructively harvested every day and the percentage of WS-like genotypes determined by spreading onto KB plates and examin- ing colony morphologies. Means ts.e. are shown (n 5). WS-like genotypes were not observed in the negative control (shaken microcosms under normal and low O2 conditions) (data not shown).

Explanation / Answer

After excluding day 0, normal and low O2 level 4 static microcosms and 4 higher O2 level at the A-L( air-liquid) interface microcosms require for four days incubation, because WS like genotype were detectable after 24 for both condition and each day incubated microcosms would be distructivaly cultivated for KB culture so four days require total 8 microcosms.

So the answer will be 8 microcosms.

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