Fill out the dilution factors below each tube and calculate the number of cells
ID: 149624 • Letter: F
Question
Fill out the dilution factors below each tube and calculate the number of cells per milliliter as well as the number of cells in he entire culture. Fill out the dilution factors below each tube and calculate the number of cells per milliliter as well as the number of cells in he entire culture. Fill out the dilution factors below each tube and calculate the number of cells per milliliter as well as the number of cells in the entire culture. A. jal 274 /1 x 10,5; Col Cells in the entire culture: B. Cells/ml: Cells in the entire culture:Explanation / Answer
A) Stock: 2.74 X 107 cells/ml; Total cells in culture is 2.74 X 107 cells/ml X 9 ml = 2.46 X 108
10-1: 2.74 X 106 cells/ml; Total cells in culture is 2.74 X 106 cells/ml X 9 ml = 2.46 X 107
10-2: 2.74 X 105 cells/ml; Total cells in culture is 2.74 X 105 cells/ml X 9 ml = 2.46 X 106
10-3: 2.74 X 104 cells/ml; Total cells in culture is 2.74 X 104 cells/ml X 9 ml = 2.46 X 105
10-4: 2.74 X 103 cells/ml; Total cells in culture is 2.74 X 103 cells/ml X 9 ml = 2.46 X 104
10-5: 2.74 X 102 cells/ml; Total cells in culture is 2.74 X 102 cells/ml X 9 ml = 2.46 X 103
10-6: 2.74 X 101 cells/ml; Total cells in culture is 2.74 X 101 cells/ml X 9 ml = 2.46 X 102
B) Stock: 1.42 X 109 cells/ml; Total cells in culture is 1.42 X 109 cells/ml X 4 ml = 5.68 X 109
10-2: 1.42 X 107 cells/ml; Total cells in culture is 1.42 X 107 cells/ml X 99 ml = 1.4 X 109
10-3: 1.42 X 106 cells/ml; Total cells in culture is 1.42 X 106 cells/ml X 9 ml = 1.28 X 107
10-4: 1.42 X 105 cells/ml; Total cells in culture is 1.42 X 105 cells/ml X 9 ml = 1.28 X 106
10-5: 1.42 X 104 cells/ml; Total cells in culture is 1.42 X 104 cells/ml X 9 ml = 1.28 X 105
10-6: 1.42 X 103 cells/ml; Total cells in culture is 1.42 X 103 cells/ml X 9 ml = 1.28 X 104
10-7: 1.42 X 102 cells/ml; Total cells in culture is 1.42 X 102 cells/ml X 9 ml = 1.28 X 103
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