1) You use the Bradford (coomassie) assay to determine the concentration of a wh
ID: 149297 • Letter: 1
Question
1) You use the Bradford (coomassie) assay to determine the concentration of a whey protein. You use bovine albumin as your standard. You determine the histone protein concentration to be 3 mg/ml. When you use the same whey protein as your standard, you determine the histone protein concentration to be 5 mg/ml. Explain the discrepancy (pick ONE answer): (2 points) The histone protein has fewer basic amino acids than albumin. The histone protein has more acidic amino acids than albumin c. The histone protein has more basic amino acids than albumin d. The histone protein contains more methionine than albuminExplanation / Answer
the answer is C.
Because of the Bradford assay is used to estimate the protein concentration by the formation of the protein-dye complex to the basic amino acid residues, arginine, lysine, and histidine. this assay method used actually to measure the presence of these basic amino acids. Histidine has more basic amino acids rather than in albumin, that's why more protein-dye complex formed in the case of the histone protein, more protein-dye complexes are formed.
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