5-7 5. Which technology can be used to detect expression of certain RNA in a tis

Question

5-7 5. Which technology can be used to detect expression of certain RNA in a tissue? (A) Southern blotting (B) Northern blotting (C) western bloting (D) immunoprecipitation 6. To clone the human insulin gene into a plasmid, which of the following is NOT needed? (A) Restriction enzymes (B) DNA ligase (C) Markers (D) Antibodies 7. Which of these secondary antibodies can be used in a western blottng for a primary antibody produced in rabbit? (A) Rabbit anti-mouse (B) Goat anti-rabbit (C) Goat anti-mouse (D) Rabbit anti-rabbit

Explanation / Answer

5. B) Northern blogging can be used to detect expression of certain RNA in a tissue.

It is a technique in which RNA bands are blog transferred from the gel onto chemically reactive paper(amino benzyl oxymethyl cellulose)

6.d) To clone the human insulin gene into a plasmid,antibodies is not needed.

Because to clone the human insulin gene into a plasmid we need a single restriction site,selective marker and foreign DNA.

7.a) Rabbit anti-mouse can be used in a western blotting for a primary antibody produced in rabbit.

It is a technique to detect the proteins of a particular specificity.

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