View Insert Format Tools Table Window Help BIOL 460 Exam 1 FINAL 2018 [Compatibi

Question

View Insert Format Tools Table Window Help BIOL 460 Exam 1 FINAL 2018 [Compatibility Mode) nsert Design Layout References Mailings Review View Sha Cambria (Body). .11-. ng A- ·-·-·, T A- A- styles Styles Pane 44. A noncoding SNP is as follows (only a portion of the flanking sequence is shown), with 60% possessing sequence A, 40% possessing sequence B; assaying a population of affected individuals and normal counterparts could be most quickly performed using a combination of which molecular approaches? A.GCATCCAAATGAATTCTAGCCTTGCA... B. GCATCCAAATGATTTCTAGCCTTGCA.. a. PCR cloning and Sanger sequencing b. endonuclease digestion and Southern blotting c. PCR followed by endonuclease digestion d. fluorescent labeling followed by comparative genomic hybridization e. none of the above

Explanation / Answer

Answer: Option C

Explanation:

A Sequence: G C A T C C A A A T G A A T T C T A G C C T T G C A

B Sequence: G C A T C C A A A T G A T T T C T A G C C T T G C A

The above mentioned "A" sequence has GAATTC restriction site which can be recognised and cleaved by EcorI restriction endonuclease. When there is a single nucleotide polymorphism in the restriction site, EcorI restriction endonuclease can not recognise and cleave the respective sequence "B". Hence PCR followed by endonuclease digestion would be the easiest and fastest way to detect the variations among the respective given sequences.

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