y tne PINE adjustment knob, slowly adjust until the picture is in focus again O.

Question

y tne PINE adjustment knob, slowly adjust until the picture is in focus again O. Fine adjust until the sample comes into focus QUESTION 8 12.5p iven that resolving power is calculated as D, the minimum distance that two points can be resolved, what s the resolving power when blue light (-471 the numerical aperture of the condenser is 125 nanometers, and the numer cal aperture of the objective lens is 0.7 Report your answer in nanometers rounded to the nearest tenth of a decimal point Click Save and Submit to save and submit. Click Save All Answers to save all ansuvers 2 3

Explanation / Answer

The term ‘resolution’ or resolving power is used to describe the ability of a microscope to differentiate details in an object when viewed by the user. It is linked to the wavlength, and the numerical aperture of the objective and condensor systems.

Using the  Rayleigh equation,

R= 1.22 /NAobj+NAcond

NA = (1.25+0.7) = 1.95nm

=471nm

resolving power = (1.22x471)/ 1.95

=294.676 nm or 294.7nm

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