1. To grow our bacteria that contain the ampicillin resistance plasmid, we use t
ID: 148667 • Letter: 1
Question
1. To grow our bacteria that contain the ampicillin resistance plasmid, we use the e will use a stock solution of 13mg/mL. antibiotic ampicillin (AMP) in our LB. W The final concentration of the antibiotic in the LB broth should be 100ug/ml a. First, convert 100/mL to mg/mL b. If the final concentration of ampicilin is 100ug/mL, then what volume of the 13mg/mL ampicillin stock mentioned above is required to make 327mL of this LB broth + AMP? Show all your work for full credit and include the correct units where applicable. 2. In Lab 2A, we streaked plates for isolation. This week, in Lab 2B, we will pick a colony off one on those plates, the MM294/pAMP on the LB/AGAR +AMP plate, and grow only bacteria from this 1 colony a. In 3-5 sentences, BRIEFLY explain why we will need to grow this colony up in the presence of ampicillin and not just in the LB broth alone. In other words, what role does the ampicillin play? What would happen if the ampicillin was not present?Explanation / Answer
1.a. calculation done in image. Ans is 0.1mg/ml
b. 2.515 ml stock will be required.
2.a.
- In only LB broth all those bacteria will grow which ever present in the sample.
- In presence of ampicilin the only bacterial population resistance to ampicilin will grow.
- since we know our clone is resostance to ampicilin wr can identify and grow our cloned bacteria separately.
-Ampicilin plays as a screening agent which identifies the cloned ones from the non transformed cells.
-If ampicilin is absent we can not screen the transformed and non transformed cells.
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