40x brightfiel 40x phase contrast PHASE SPECIMEN In this part of the exercise, y

Question

40x brightfiel
40x phase contrast PHASE SPECIMEN In this part of the exercise, you will observe unstained cheek epithelial cells with bright field and phase contrast microscopy. 1, Place a drop of distilled water on a clean glass slide. Using a sterile micropipette tip, gently scrape the inside of your cheek (squamous epithelial cells). 2. Place the scrapped epithelial cells in the drop of water on the slide and mount a clean coverslip over the specimen. 3. Examine the cells under 10X bright field microscopy. Adjust for Koehler illumination with each objective. Switch to 40X bright field, adjust for Koehler illumination, and draw a representative cell in the space provided below 4. Switch to 40X phase microscopy by rotating the condenser to the "40" position on the condenser, and draw a representative cell below. 40X BRIGHTFIELD 40X PHASE CONTRAST a. Compare and contrast an amplitude object with a phase object. b. What cellular components are more clearly delineated with phase than with bright field microscopy when viewing cheek epithelial cells?

Explanation / Answer

Answer:

a) on comparing the amplitude object with the phase object, the phase object is much clearly visible compared to the amplitude object.

b) The epithelial cells look squamous shaped with visible nucleus and cell membrane in the phase compare to bright field in which it looks like irregular shape.

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