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Nucleotides, Nucleic Acids and Proteins: Structure and Function 27 Experiment 1:

ID: 148459 • Letter: N

Question

Nucleotides, Nucleic Acids and Proteins: Structure and Function 27 Experiment 1: Enzyme Function (con't) The rate of enzyme efficiency can be affected by a number of factors. In this tab, you will experimentally alter the availability of reactants, pH, and temperature. Enzyme efficiency relative to availability of reactants You will examine the effect of the availability of substrates, by comparing the relative change in brownness by comparing two apples with different surface areas: one sliced and ane grated. 14. Finish this hypothesis about the rate of enzyme-mediated synthesis of benzoquinone and the surface area of exposed fruit. Fruit tissue with higher exposed surface area will. How does increasing surface area affect the availability of substrates? Which substrate is limiting? Enzyme efficiency relative to pH Enzymes are pH specific, meaning they work most efficiently with an optimal range of pH values. Above or below an enzyme's optimal range can cause the enzyme to denature laltering their 3-D structurel. and therefore lose their functionality. You will compare the relative change in brownness by comparing two apple slices immersed in two different pH solutions: neutral (H,01 and acidic (lemon juice) 15. Finish this hypothesis about the rate of enzyme-mediated synthesis of benzoquinone and pH. Fruit tissue in an acidic solution will.. What logic did you use in generating your hypothesis? Enzyme efficiency relative to temperature Enzymes also have a specific optimal range of temperatures. Similar to ph, temperatures above or below an enzyme's optimal range will cause the protein to denature, impeding their functionality 16. Finish these hypotheses about the rate of enzyme-mediated synthesis of benzoquinone and temperature (relative to room temperature). Fruit tissue exposed to warm temperature will. Fruit tissue exposed to cold temperature will..

Explanation / Answer

1)Fruit tissue with higher exposed surface area will increase the enzyme activity on it by benzoquinone

because increasing the surface area increases the substrate by increasing the enzyme acitivity.The fruit tissue will be limiting because benzoquinine will increase and the enzyme acitivity over the fruit will increase overall.

2)Fruit tissue in an acidic solution will exhibit enzymatic browning because tissue of the fruit are crushed by the high pH of the acid which turns the fruit brown.The enzyme acitivity of acid on the fruit increases.The rate of enzyme mediated synthesis of benzoquine increases because of an increase in the enzyme activity which increases due to an increase in pH.

3)Fruit tissue exposed to warm temperature will increase the enzyme mediated synthesis of benzoquinone because the enzyme becomes active which leads to killing of the plants at such high temperatures.

Fruit tissue exposed to cold temperature inhibits the enzyme mediates symthesis of benzoquinone because in cold weather the enzyme becomes inactive which inhibits its performance completely.