These two diagrams show two different ways of looking at a membrane protein call

Question

These two diagrams show two different ways of looking at a membrane protein called the beta-adrenergic receptor, which spans the membrane 7 times. This receptor, which we will talk more about soon, is on the surface of your muscle cells, where it converts the "fight or flight" signal of adrenaline in your blood stream into enhanced muscle metabolism to power your frantic escape from saber-toothed tigers, angry roommates, etc. The first diagram is a simple primary structure (amino acid sequence), with the residues numbered N-terminus to C-terminus, from 1 to 413, and the membrane-spanning regions in red. The numbering system is simple: the zero in "10" sits directly above the single-letter abbreviation for the 10th residue, the zero in "20" sits above the 20th residue, etc. The second is a 2-D representation of how the beta-adrenergic receptor is actually organized in the plasma membrane of your muscle cells, with the amino acid residues labeled at the boundaries between membrane spans and cytoplasmic or extracellular portions Beta-2 adrenergic receptor, Homo sapiens (i) primary sequence 60 MGQPGNGSAF LLAPNRSHAP DHDVTQQRDE VWVVGMGIVM SLIVLAIVFG NVLVITAIAK 20 30 40 50 120 FERLQTVTNY FITSLACADL VMGLAVVPFG AAHILMRMIWT FGNFWCEFWI SIDVLCVTAS 70 80 90 100 110 180 IETLCVIAVD RYFAITSPFK YQSLLTKNKA RVIIIMVWIV SGLTSFLPI MHWYRATHQE 130 140 150 160 170 240 AINCYANETC CDFFTNQAYA IASSIVSFYV PLVIMVFVYS RVFQEAKRQL QKIDKSEGRF 190 200 210 220 230 300 HVQNLSQVEQ DGRTGHGLRR SSKFCLKEHK ALKTLGIIMG TFTLCWLPFF IVNIVHVIQD 250 260 270 280 290 3 60 NLIRKEVYIL LNWIGYVNSG FNPLIYCRSP DFRIAFQELL CLRRSSLKAY GNGYSSNGNT 310 320 330 340 350 370 380 3 90 400 410 GEQSGYHVE EKENKLLCED LPGTEDFVGH QGTVPSDNID SQGRNCSTND SLL iin 2-D arrangement in memhrane

Explanation / Answer

For a positive result in fluorescence microscopy, the antibody must bind to the sequence on the extracellular phase.

In order to identify the correct sequence to which an antibody can possibly bind, the given sequences are located on the 2D structure with the help of their amino acid sequence numbers.

Option 1 sequence FERLQTVTNY (71-80) lies on the cytosolic domain of the cell. Antibody cannot bind

Option 2 sequence YQSLLTKNKA (141-150) lies on the cytosolic domain of the cell. Antibody cannot bind.

Option 3 sequence AINCYANETC (181-190) lies on the extra cellular domain of the cell. Antibody can possibly bind.

Option 4 sequence SQGRNCSTND (401-410) lies on the cytosolic domain of the cell. Antibody cannot bind.

Option 5 sequence ALKTLGIIMG (271-280) lies partly on the cytosol and partly on the trans-membrane domain. Antibody cannot bind.

Option 6 sequence SLIVLAIVFG (41-50) lies completely within the transmembrane domain. antibody cannot bind.

Hence, it can be said that the sequence AINCYANETC (181-190) binds to the antibody.

Answer :Option 3 sequence AINCYANETC (181-190)

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