1. Which of the following statements best describes how the Gram stain allows yo

Question

1. Which of the following statements best describes how the Gram stain allows you to differentiate between different bacterial cells? a) The Gram stain distinguishes between bacteria that either have or lack a cell membrane b) The Gram stain distinguishes between bacteria that either have or lack a cell wall c The Gram stain distinguishes between bacteria that either have or lack peptidoglycan in their cell wall d) The Gram stain distinguishes between bacteria that have different thicknesses of peptidoglycan in their cell wall e) The Gram stain distinguishes between bacteria that have different thicknesses of cell membranes 2. What is the correct order of the reagents used in during Gram staining (from start to finish)? a) Safranin, Gram's lodine, Crystal Violet, Ethanol b) Gram's Iodine, Crystal Violet, Safranin, Ethanol c) Crystal Violet, Gram's lodine, Safranin, Ethanol d) Crystal Violet, Gram's lodine, Ethanol, Safranin Safranin, Crystal Violet, Gram's lodine, Ethanol 3. During a Gram staining and Microscopy experiment, a student records the colours of microbial cells following Gram stains as follows: Isolate 1: Purple; Isolate 2: Pink. What would be the Gram stain reactions for these two isolates? a) Isolate l : Gram Positive and isolate 2: Gram Positive b) Isolate: Gram Negative and Isolate 2: Gram Positive c) Isolate : Gram Positive and Isolate 2: Gram Negative d) Isolate 2: Gram Negative and Isolate 2: Gram Negative e) Isolate1: Fungus and Isolate 2: Bacterium What would be the overall magnification of microbial cells viewed using a 40X objective and 10x ocular lenses using a compound microscope? a) 40x b) 10x c) 400x d) 50x e) 500x

Explanation / Answer

1) d

2)d) instead of ethanol, acetone can also be used

3) c)

4)c)

5)c

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